J. Schwamborn, H.Schöler, P.Ertl, V.Bonifati, D.Seliktar
3DPD: Advanced modelling of Parkinsons disease with three-dimensional human midbrain organoids
Parkinson's disease & PD-related disorders
One of the main limitations in neuroscience and in the modeling of neurodegenerative diseases is the lack of advanced experimental in vitro models that truly recapitulate the complexity of the human brain. Therefore, it is the aim of this research project to generate brain-like structures, so called organoids, that resemble the human midbrain. These organoids will be developed in a multifunctional lab-on-a-chip device. We call this a midbrain-on-a-chip approach. We will focus on Parkinsons disease, which is the second most common neurodegenerative dis- ease. The midbrains-on-a-chip will be generated from induced pluripotent stem cells derived from Parkinsons disease patients. This will allow us to elucidate how Parkinsons disease imparts architectural remodelling, dopamine release and network formation of the midbrain tissue. The successful cultivation of in vitro midbrain organoids in a micro-analytical analysis platform will yield substantial insights and open new avenues for exploring the mechanisms of onset and pro- gression under physiologically relevant measurement conditions. Moreover, with the usage of mi- crofluidics devices, the whole approach is cost-effective and suitable for screening purposes.