Studies on the senescent human noradrenergic system with the novel iDISCO 3D immuno-imaging method with special attention on Alzheimers disease
The Swedish Brain Foundation
The aim of this application is to (i) explore the neurochemical features and innervation of subdivisions of the human LC; (ii) examine the morphology of human noradrenergic axon arborization with special attention to the temporal and spatial distribution of the aberrant noradrenergic structures we
described recently; (iii) explore the microenvironment of these fiber aberrations in the neocortex; (iv) 4 explore the genome-wide mRNA expression-profile of Sstr2-/- mice in the neocortex or LC neurons to determine genotype-specific expression patterns that could explain the selective noradrenergic axonal
The main goal of these planned studies is (i) to adjust a high power immune-imaging technique for the human brain that could be useful to obtain significant new information applicable in further therapeutical interventions based on neuromodulation; (ii) to determine neurochemical targets that could explain the intrinsic selectivity of degeneration among human LC neurons during normal senescence and
pathological conditions of early and advanced stages of Alzheimers disease and (iii) to determine (local) factors in the human neocortex that facilitate/inhibit the degeneration or potential sprouting of noradrenergic fibers.